Genetic stability (in vivo) of the attenuated oral rabies virus vaccine SAD B19.
Identifieur interne : 000167 ( PubMed/Checkpoint ); précédent : 000166; suivant : 000168Genetic stability (in vivo) of the attenuated oral rabies virus vaccine SAD B19.
Auteurs : Aline Beckert [Allemagne] ; Lutz Geue ; Ad Vos ; Andreas Neubert ; Conrad Freuling ; Thomas MüllerSource :
- Microbiology and immunology [ 0385-5600 ] ; 2009.
English descriptors
- KwdEn :
- Administration, Oral, Animals, Base Sequence, Cell Line, Cricetinae, Foxes, Genomic Instability, Mice, Molecular Sequence Data, Mutation, Rabies (virology), Rabies Vaccines (administration & dosage), Rabies Vaccines (genetics), Rabies virus (genetics), Rabies virus (pathogenicity), Serial Passage, Vaccines, Attenuated (administration & dosage), Vaccines, Attenuated (genetics), Viral Proteins (genetics), Virulence.
- MESH :
- chemical , administration & dosage : Rabies Vaccines, Vaccines, Attenuated.
- chemical , genetics : Rabies Vaccines, Vaccines, Attenuated, Viral Proteins.
- genetics : Rabies virus.
- pathogenicity : Rabies virus.
- virology : Rabies.
- Administration, Oral, Animals, Base Sequence, Cell Line, Cricetinae, Foxes, Genomic Instability, Mice, Molecular Sequence Data, Mutation, Serial Passage, Virulence.
Abstract
The distribution of oral rabies vaccine baits containing replication-competent live viruses poses certain environmental safety risks; among others, the possibility of reversion to or an increase in virulence. Hence, the genetic stability of the complete genome of the most widely used oral rabies vaccine virus, SAD B19, was examined after four and 10 serial i.c. passages in foxes and mice, respectively. It was shown that the consensus strain of SAD B19 was extremely stable in vivo. After 10 consecutive passages in mice not a single mutation was observed. In foxes, seven single nucleotide exchanges were found between the first and fourth passage, of which only one resulted in an amino acid exchange at position 9240 of the L-gene. This mutation was not observed during the first three passages and, furthermore, it was shown that this mutation was not linked to enhanced virulence.
DOI: 10.1111/j.1348-0421.2008.00085.x
PubMed: 19161553
Affiliations:
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possibility of reversion to or an increase in virulence. Hence, the genetic stability of the complete genome of the most widely used oral rabies vaccine virus, SAD B19, was examined after four and 10 serial i.c. passages in foxes and mice, respectively. It was shown that the consensus strain of SAD B19 was extremely stable in vivo. After 10 consecutive passages in mice not a single mutation was observed. In foxes, seven single nucleotide exchanges were found between the first and fourth passage, of which only one resulted in an amino acid exchange at position 9240 of the L-gene. This mutation was not observed during the first three passages and, furthermore, it was shown that this mutation was not linked to enhanced virulence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19161553</PMID><DateCreated><Year>2009</Year><Month>01</Month><Day>23</Day></DateCreated><DateCompleted><Year>2009</Year><Month>03</Month><Day>10</Day></DateCompleted><DateRevised><Year>2009</Year><Month>01</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0385-5600</ISSN><JournalIssue CitedMedium="Print"><Volume>53</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Microbiology and immunology</Title><ISOAbbreviation>Microbiol. 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In foxes, seven single nucleotide exchanges were found between the first and fourth passage, of which only one resulted in an amino acid exchange at position 9240 of the L-gene. This mutation was not observed during the first three passages and, furthermore, it was shown that this mutation was not linked to enhanced virulence.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Beckert</LastName><ForeName>Aline</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Epidemiology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Seestrasse 55, 16868 Wusterhausen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Geue</LastName><ForeName>Lutz</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Vos</LastName><ForeName>Ad</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Neubert</LastName><ForeName>Andreas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Freuling</LastName><ForeName>Conrad</ForeName><Initials>C</Initials></Author><Author 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A" first="Ad" last="Vos">Ad Vos</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Beckert, Aline" sort="Beckert, Aline" uniqKey="Beckert A" first="Aline" last="Beckert">Aline Beckert</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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